High resolution NMR in biofluids and tissues

U. Eichhoff^ab, M. Spraul^b, M. Piotto<sup>c

a</sup> Moscow State University
^b "Bruker BioSpin", Rheinstetten, Germany
^c "Bruker BioSpin", Wissembourg, France

Abstract: Bodyfluids are complicated mixtures and each component has its own NMR spectrum. The total spectrum may contain several thousand lines, but with the help of modern homo- and heteronuclear multidimensional NMR methods all lines can be assigned and a metabolite data base can be created. A principal component analysis of all spectra makes it possible to reveal deviation from the normal state. Spectra corresponding to this deviation then have to be evaluated more carefully using spectra simulation and data base programs. Tissues are heterogenous samples and in normal NMR spectra exhibit comparably broad lines due to a residual dipole coupling. Magic angle spinning (MAS) averages out these interactions leading to well resolved spectra which allow the identification of various metabolites. Metabolic changes due to malignant diseases can be detected, which is the basis of NMR-histology, a new discipline that has already been applied in various hospitals.

Keywords: NMR, magic angle spinning, NMR-histology, oncology, tissues, biofluids.

UDC: 543.429.23:616-073.584

Received: 01.02.2012