Abstract:
“Crude” extracts are a new perspective type of bioelectrocatalysts for biofuel cells. The method of obtaining a “crude” extract by disintegrating bacterial cell membranes (for example, by ultrasound) is simpler and more economical in comparison with pure enzymes. From a scientific point of view, the study of the biocatalytic properties of “crude” extracts is very interesting, since the disintegrated biomass contains a cascade of enzymes involved in the metabolism of microorganisms and coenzymes necessary for oxidative/reductive transformation. This set of bioactive substances makes it possible to simulate the processes occurring during the operation of a “natural” fuel cell in an artificial environment. The effect of the pH and composition of the LB nutrient medium used in the cultivation of E.coli on the dehydrogenase activity of protein extracts obtained from this culture was studied. It is shown that by selecting the pH and modifying the composition of the LB nutrient medium, it is possible to increase the value of the specific dehydrogenase activity of extracts by almost 9 times (from 0.25 to 2.2 mg/mg).
First page:
PDF ôàéë