Development of technology for obtaining a new bioelectrocatalyst - “crude” extract of Saccharomyces cerevisiae

M. V. Dmitrieva^a, V. A. Pavlov^ba, P. S. Afanasyeva^ab, E. V. Zolotukhina<sup>c

a</sup> Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Moscow region
^b Lomonosov Moscow State University
^c Moscow Institute of Physics and Technology

Abstract: “Crude” extracts obtained by ultrasonic disintegration of microorganisms are promising bioelectrocatalysts. Extracts from Saccharomyces cerevisiae are of particular interest because they may contain a significant number of enzyme systems that oxidize simple carbohydrates such as glucose. A technology for producing “crude” protein extracts of Saccharomyces cerevisiae has been developed. The percentage of glucose added to the nutrient medium, as well as the composition of the buffer solution at the resuspending stage, the power and time of ultrasonic disintegration of biomass, the rate and time of centrifugation during separation of the sediment of destroyed cells from the filler fluid have a significant effect on the enzymatic activity of the resulting “crude” extract.

UDC: 663.15, 66.098

Received: 07.06.2023
Accepted: 19.02.2024

DOI: 10.18522/1026-2237-2024-1-133-140

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