This article is cited in 5 papers

Laser applications and other topics in quantum electronics

Measurement of diffusion of fluorescent molecules in living cells

S. A. Tatarkova^ab, C. Lloyd^c, D. Berk<sup>c

a</sup> Saratov State University
^b School of Physics and Astronomy, University of St. Andrews, North Haugh St. Andrews, Scotland
^c School of Pharmacy, Coupland III, University of Manchester, UK

Abstract: The possibilities of the method of fluorescence correlation spectroscopy for studying the molecular dynamics in living cells are demonstrated. The method provides point measurements of extremely low concentrations of fluorescent molecules and their diffusion coefficients with a high time resolution in a microscopic volume, which is especially important in pharmacological investigations. A biological model of the interaction of liposomes with a cellular membrane is considered. The diffusion coefficients of fluorescent molecules are measured directly in the living cell cytoplasm.

PACS: 87.50.Hj, 87.64.-t

Received: 21.05.2002
Revised: 16.10.2002

 English version: Quantum Electronics, 2003, 33:4, 357–362

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