This article is cited in 51 papers

Laser applications and other topics in quantum electronics

Spectral-phase interference method for detecting biochemical reactions on a surface

P. I. Nikitin^a, B. G. Gorshkov^a, M. V. Valeiko^a, S. I. Rogov<sup>b

a</sup> A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Mosscow
^b M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow

Abstract: A simple optical method is proposed for the direct detection of biochemical reactions on a surface, which is insensitive to variations in the emission intensity and refractive index of a solution. The method is based on the detection of the spectrum of reflected or transmitted emission modulated by the interference in a sensitive layer of a large thickness (several tens and hundreds of microns), which can be a microscope cover glass with a deposited receptor layer. A change in the phase of the interference pattern in this spectrum is used as an information signal about a change in the thickness of the sensitive layer caused by a biochemical reaction. The method was tested in studies of the reactions of binding and detachment of proteins in real time. The root-mean-square noise of the method expressed in the layer thickness is 3 pm.

PACS: 42.62.Be, 42.30.Rx

Received: 10.10.2000

 English version: Quantum Electronics, 2000, 30:12, 1099–1104

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