This article is cited in 17 papers

Laser biophotonics

Monitoring of chlorin-based photosensitiser localisation with dual-wavelength fluorescence imaging: numerical simulations

A. V. Khilov, D. A. Kurakina, I. V. Turchin, M. Yu. Kirillin

Institute of Applied Physics of the Russian Academy of Sciences, Nizhny Novgorod

Abstract: An approach to the estimation of chlorin-based photosensitiser localisation with dual-wavelength fluorescence imaging after topical application or intravenous injection is developed based on numerical simulations. Fluorescence imaging, which is commonly used for the monitoring of photosensitiser distribution within tissue, does not allow for its depth-resolved imaging. Chlorin-based photosensitisers feature two peaks in their absorption spectra corresponding to 402 and 662 nm, which allows for fluorescence excitation at two wavelengths with significantly different light penetration depths into biotissues. The ratio of fluorescence signals detected upon irradiation at different excitation wavelengths is shown to provide the information about photosensitiser localisation. The cases of exponential in-depth decay of the photosensitiser concentration and uniform photosensitiser distribution within the layer at a certain depth within the medium are analysed. These cases simulate topical application of photosensitiser and its intravenous injection and further accumulation in tumour, respectively.

Keywords: Monte Carlo simulation, fluorescence imaging, chlorinbased photosensitiser, photodynamic therapy.

Received: 05.10.2018
Revised: 09.11.2018

 English version: Quantum Electronics, 2019, 49:1, 63–69

Bibliographic databases:

• 
• 
•