This article is cited in 3 papers

Laser methods in biology

Thermal denaturation of egg protein under nanosecond pulsed laser heating of gold nanoparticles

Yu. P. Meshalkin^ab, I. N. Lapin^c, V. A. Svetlichnyi<sup>c

a</sup> Novosibirsk State Technical University
^b Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk
^c V.D. Kuznetsov Siberian Physicotechnical Institute at Tomsk State University

Abstract: Thermal denaturation of egg protein in the presence of gold nanoparticles via their heating at the plasmon resonance wavelength by the pulsed radiation of the second harmonic of an Nd:YAG laser (532 nm) is investigated. The experimental dependence of the protein denaturation time on the mean laser power is obtained. The heating temperature of the medium with gold nanoparticles is calculated. The numerical estimates of the temperature of the heated medium containing protein and gold nanoparticles (45.3°C at the moment of protein denaturation) are in good agreement with the literature data on its thermal denaturation and with the data of pyrometric measurements (42.0 ± 1.5°C). The egg protein may be successfully used to investigate the specific features of laser heating of proteins in the presence of metal nanoparticles under their excitation at the plasmon resonance wavelength.

PACS: Be, 42.55.Rz, 87.19.Ph, 65.80.+n

Received: 24.02.2011
Revised: 06.06.2011

 English version: Quantum Electronics, 2011, 41:8, 754–758

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