A. N. Morozov, A. A. Lazutkin, I. V. Turchin, V. A. Kamensky, I. I. Fiks, D. V. Bezryadnov, A. A. Ivanova, D. M. Toptunov, K. V. Anokhin, “Fibreoptic fluorescent microscopy in studying biological objects”, Kvantovaya Elektronika, 2010, Volume 40, Number 9,Pages <nobr>842

This article is cited in 6 papers

Fluorescent ultramicroscopy

Fibreoptic fluorescent microscopy in studying biological objects

A. N. Morozov^a, A. A. Lazutkin^b, I. V. Turchin^a, V. A. Kamensky^a, I. I. Fiks^a, D. V. Bezryadnov^b, A. A. Ivanova^b, D. M. Toptunov^b, K. V. Anokhin^b

^a Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod
^b NII of Normal Physiology of RAMS

Abstract: The method of fluorescent microscopy is developed based on employment of a single-mode fibreoptic channel to provide high spatial resolution 3D images of large cleared biological specimens using the 488-nm excitation laser line. The transverse and axial resolution of the setup is 5 and 13 μm, respectively. The transversal sample size under investigation is up to 10 mm. The in-depth scanning range depends on the sample transparency and reaches 4 mm in the experiment. The 3D images of whole mouse organs (heart, lungs, brain) and mouse embryos obtained using autofluorescence or fluorescence of exogenous markers demonstrate a high contrast and cellular-level resolution.

PACS: 87.64.M-, 42.81.Wg, 33.50.-j

Received: 19.04.2010
Revised: 08.07.2010