I. V. Kukhtevich, K. I. Belousov, A. S. Bukatin, M. V. Dubina, A. A. Evstrapov, “A microfluidic chip with hydrodynamic traps for <i>in vitro</i> microscopic investigations of single cells”, Pisma v Zhurnal Tekhnicheskoi Fiziki, 2015, Volume 41, Issue 5,Pages <nobr>103

This article is cited in 5 papers

A microfluidic chip with hydrodynamic traps for in vitro microscopic investigations of single cells

I. V. Kukhtevich^ab, K. I. Belousov^a, A. S. Bukatin^bc, M. V. Dubina^c, A. A. Evstrapov^abc

^a St. Petersburg National Research University of Information Technologies, Mechanics and Optics
^b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
^c Alferov Federal State Budgetary Institution of Higher Education and Science Saint Petersburg National Research Academic University of the Russian Academy of Sciences, St. Petersburg

Abstract: The results on making a microfluidic chip for in vitro microscopic investigations of single cells are presented. Numerical simulation of the motion trajectories of microparticles makes it possible to determine the geometry of hydrodynamic traps, their number, and the trap arrangement in a reaction chamber. According to the developed design, microfluidic chips were fabricated from a SU-8 photoresist by photolithography. The microfluidic chips have been tested to prove their operating capacity for isolating and holding K562 human myeloid leukemia cells from a sample flow and their subsequent investigation by confocal laser scanning microscopy.

Received: 20.10.2014