Fabrication of nanoscale multimolecular structures of lanthanum stearate using Langmuir monolayers for laser desorption/ionization mass spectrometry

A. Yu. Gorbunov^ab, E. P. Podolskaya<sup>c

a</sup> Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russia
^b Research Institute of Hygiene, Occupational Pathology and Human Ecology, FMBA, St. Petersburg, Russia
^c Federal State-Financed Institution Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency, St. Petersburg, Russia

Abstract: Matrix-assisted laser desorption/ionization (MALDI) from the surface of nanosized multimolecular structures based on lanthanum stearate monolayers (FLa) has been studied. The presence of FLa on the surface of MALDI target was confirmed experimentally by laser desorption of lanthanum-containing organic ions. MALDI target functionalization with FLa is shown to significantly increase the yield of target peptide ions, with the optimum being achieved at a film thickness of about 6 monolayers. An approach is proposed in a “lab-on-a-plate” format, which allows specific extraction of peptides modified with chlorine-containing compounds and includes the following steps: functionalization of the target surface, metal affinity extraction, matrix deposition and MALDI mass spectrometric analysis.

Keywords: mass spectrometry, matrix assisted laser desorption/ionization (MALDI), surface, Langmuir monolayers, metal affinity chromatography.

Received: 22.07.2022
Revised: 22.07.2022
Accepted: 15.09.2022

DOI: 10.21883/PJTF.2022.21.53711.19320

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