I. V. Kukhtevich, K. I. Belousov, A. S. Bukatin, V. I. Chubinskiy-Nadezhdin, V. Yu. Vasileva, Yu. A. Negulyaev, A. A. Evstrapov, “Microfluidic chips for the study of cell migration under the effect of chemicals”, Pisma v Zhurnal Tekhnicheskoi Fiziki, 2016, Volume 42, Issue 9,Pages <nobr>71

This article is cited in 1 paper

Microfluidic chips for the study of cell migration under the effect of chemicals

I. V. Kukhtevich^ab, K. I. Belousov^b, A. S. Bukatin^ac, V. I. Chubinskiy-Nadezhdin^d, V. Yu. Vasileva^d, Yu. A. Negulyaev^de, A. A. Evstrapov^abc

^a Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
^b St. Petersburg National Research University of Information Technologies, Mechanics and Optics
^c Federal State Budgetary Institution of Higher Education and Science Saint Petersburg National Research Academic University of the Russian Academy of Sciences, St. Petersburg
^d Institute of Cytology Russian Academy of Science, St. Petersburg
^e Peter the Great St. Petersburg Polytechnic University

Abstract: Numerical simulation of the formation of a chemoattractant gradient in reaction chambers of a chip having different geometries enabled the determination of a structure suitable for the study of cell migration, in accordance with which hybrid polymer–glass microfluidic devices were manufactured. Verification of the procedures of alignment of cells in the reaction chamber of the chip by centrifugal force and subsequent culturing of the cells showed that microfluidic chips can be used to study cell migration under the effect of the chemoattractant gradient in vitro.

Received: 16.11.2015