International Conference Physics.SPb/2024, October 21-25, 2024, St. Petersburg

Detection of single biomolecules using a solid-state nanopore SiN/Si sensor

N. V. Vaulin^a, P. K. Afonicheva^b, D. V. Lebedev^ab, A. S. Bukatin^ab, I. S. Mukhin^ac, A. A. Evstrapov<sup>b

a</sup> Alferov Federal State Budgetary Institution of Higher Education and Science Saint Petersburg National Research Academic University of the Russian Academy of Sciences, St. Petersburg
^b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
^c Peter the Great St. Petersburg Polytechnic University

Abstract: Developed a technique for immobilizing single molecules of the 26S proteasome in the region of a solid-state SiN nanopore under the action of an applied electric field. The process of immobilization of a single molecule of the 26S proteasome leads to a change in the ion current through the nanopore by 20%. Further development of this technique may allow highly efficient immobilization of single biomolecules in order to study their conformational activity.

Keywords: nanopore, solid-state nanopore, nanopore sensor, single molecule detection, 26S-proteasome.

Received: 03.05.2024
Revised: 01.07.2024
Accepted: 30.10.2024

DOI: 10.61011/FTP.2024.09.59303.6342A

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