This article is cited in 2 papers

International scientific conference "XIII International Conference on hole burning, single molecule, and related spectroscopies: science and applications (HBSM-2018)"
Biophotonics

Three-dimensional luminescence tomographic visualization of biological tissues

V. V. Rocheva^a, A. G. Savelyev^ab, A. V. Nechaev^ac, A. N. Generalova^ad, V. A. Semchishen^a, A. V. Zvyagin^be, E. V. Khaidukov<sup>ab

a</sup> FSRC "Crystallography and Photonics" RAS
^b I. M. Sechenov First Moscow State Medical University
^c Moscow Technological University, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia
^d M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow
^e Macquarie University, Sydney, Australia

Abstract: We propose a method for obtaining a fluorescence tomographic image for visualization and diagnosis of tissues of a living organism. The method is based on the excitation of the luminescence of multicolor upconverting nanoparticles localized in the depth of the biological tissue or a phantom imitating it by IR light. By recording the changes in the shape of the spectrum of the intensity of luminescence radiation from luminescent nanoparticles on the surface of the tissue, it is possible to obtain information about the depth of their occurrence. To implement this approach, upconverting nanoparticles were synthesized on the base of $\beta$-NaYF$_4$ crystal matrix doped with rare-earth elements Yb$^{3+}$, Er$^{3+}$, and Tm$^{3+}$. The luminescence spectra of the produced nanoparticles upon excitation at a wavelength of 980 nm contain three narrow bands with maxima at wavelengths 540, 655, and 800 nm.

Received: 24.09.2018

DOI: 10.21883/OS.2019.01.47060.261-18

 English version: Optics and Spectroscopy, 2019, 126:1, 92–94

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