K. Yu. Kandurova, E. V. Potapova, E. A. Zherebtsov, V. V. Dremin, E. S. Seryogina, A. Yu. Vinokurov, A. V. Mamoshin, A. V. Borsukov, Yu. V. Ivanov, A. V. Dunaev, “Testing a fine-needle optical probe for recording changes in the fluorescence of coenzymes of cellular respiration”, Optics and Spectroscopy, 2020, Volume 128, Issue 6,Pages <nobr>736

This article is cited in 5 papers

Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods
Biophotonics

Testing a fine-needle optical probe for recording changes in the fluorescence of coenzymes of cellular respiration

K. Yu. Kandurova^a, E. V. Potapova^a, E. A. Zherebtsov^ab, V. V. Dremin^ab, E. S. Seryogina^a, A. Yu. Vinokurov^c, A. V. Mamoshin^ad, A. V. Borsukov^e, Yu. V. Ivanov^fg, A. V. Dunaev^a

^a Research and Development Centre of Biomedical Photonics, Orel State University
^b University of Oulu, Oulu, Finland
^c Department of Industrial Chemistry and Biotechnology, Orel State University, Orel, Russia
^d Orel Regional Clinical Hospital
^e Problem Research Laboratory "Diagnostic Researches and Mini-invasive Technologies", Smolensk State Medical University, Smolensk, Russia
^f Federal Scientific and Clinical Center for Specialized Medical Service and Medical Technologies, Federal Biomedical Agency of Russia, Moscow, Russia
^g Central Tuberculosis Research Institute, Moscow

Abstract: A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle optical probe for use in fine-needle aspiration biopsy of liver tumors is described. To test the developed device, experimental measurements of the fluorescence of internal organs of a laboratory rat were carried out in vivo while exposing the tissue surface to mitochondrial uncoupling to induce changes in cell respiration. The results of the model experiment showed the ability of the developed channel to detect changes in fluorescence due to changes in the processes of oxidative phosphorylation of mitochondria.

Keywords: optical biopsy, fluorescence spectroscopy, mitochondrial uncouplers, NADH, FAD.

Received: 10.12.2019
Revised: 31.01.2020
Accepted: 28.02.2020

DOI: 10.21883/OS.2020.06.49405.32-20