Abstract:
The article presents the results of a study of the characteristics of first- to fourth-order derivatives of papaverine hydrochloride absorption spectra obtained using scanning spectrophotometer software. Using the example of an asymmetric shortwave absorption band, the transformation of zero-order spectra upon differentiation with various $\Delta\lambda$ intervals from 1 to 80 nm is graphically demonstrated. The influence of the specified parameters (scanning step and differentiation interval) on the shape of the derivatives is assessed. It is established that with increasing $\Delta\lambda$, the curve shapes, positions of extrema, and “zero crossings” change. The obtained results can be used to develop methods for the identification and quantification of individual compounds and the analysis of multicomponent systems. The use of the spectrophotometer software allows for the rapid and reliable selection of optimal scanning parameters and differentiation of absorption spectra.
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