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JOURNALS // Optics and Spectroscopy // Archive

Optics and Spectroscopy, 2025 Volume 133, Issue 7, Pages 790–798 (Mi os1956)

Biophotonics

Laser diffractometry and measurement of the width of red blood cell size distribution

S. Yu. Nikitina, E. G. Tsybrovb, M. S. Lebedevaa

a Lomonosov Moscow State University, Faculty of Physics
b Lomonosov Moscow State University, Faculty of Computational Mathematics and Cybernetics

Abstract: A new algorithm for measuring the width of erythrocyte size distribution based on the analysis of the diffraction pattern occurring in the far zone during the scattering of the laser beam on a blood smear is proposed. The algorithm is applicable to the analysis of weakly heterogeneous blood samples with symmetric size distribution function. The input data for the algorithm are the characteristics of the angular distribution of light intensity near the first dark ring in the diffraction pattern. The peculiarity of the algorithm is the reduced sensitivity to diffraction pattern noise, which is important for its experimental realization.

Keywords: laser diffractometry, blood smear, measurement of the width of red blood cell size distribution.

Received: 31.07.2024
Revised: 22.04.2025
Accepted: 29.04.2025

DOI: 10.61011/OS.2025.07.61114.6944-25



© Steklov Math. Inst. of RAS, 2026