Optics and Spectroscopy, 2025 Volume 133, Issue 5,Pages 551–559(Mi os1926)
Proceedings of The XXVIII Annual International Conference "Saratov Fall Meeting 2024", September 23-27, 2024 Biophotonics
Numerical study of the influence of oxyhemoglobin, deoxyhemoglobin, and methemoglobin content on the reflection, absorption, and transmission spectra of human blood
Abstract:
The Monte Carlo method was used to numerically investigate the effect of human blood oxygenation and methemoglobin content on the reflection, absorption, and transmission spectra in the wavelength range of 400–1100 nm. It was established that replacing oxyhemoglobin with deoxyhemoglobin causes major spectral changes in the reflection, absorption, and transmission within the wavelength ranges of 450–520 nm, 590–780 nm, and 780–1100 nm, while replacing deoxyhemoglobin with methemoglobin leads to significant changes in the ranges of 520–590 nm, 590–780 nm, and 780–1100 nm. It was found that within the 520–590 nm range, with a peak at 580 $\pm$ 5 nm, replacing oxyhemoglobin with deoxyhemoglobin does not significantly alter reflection, absorption, or transmission, whereas changes in methemoglobin content result in an increase in reflection and transmission in this spectral range while decreasing absorption. Numerical analysis demonstrated that decreasing blood oxygen saturation leads to increased transmission (optical clearing) in the 450–520 nm and 780–1100 nm wavelength ranges, while the presence of methemoglobin causes optical clearing in the 520–590 nm range.
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