Proceedings of The XXVIII Annual International Conference "Saratov Fall Meeting 2024", September 23-27, 2024
Biophotonics

Fluorescence spectroscopy of blood plasma and cerebrospinal fluid for the diagnosis of cerebral gliomas

P. K. Nurgalieva^a, O. P. Cherkasova^bc, B. P. Yakimov^ad, E. V. Stupak^e, V. M. Glotov^e, V. V. Stupak^e, E. A. Shirshin<sup>a

a</sup> Lomonosov Moscow State University
^b Institute of Automation and Electrometry, Siberian Branch of Russian Academy of Sciences, Novosibirsk
^c Novosibirsk State Technical University
^d Laboratory of Clinical Biophotonics, Sechenov First Moscow State Medical University
^e Novosibirsk Research Institute of Traumatology and Orthopedics

Abstract: This study investigated the capabilities of fluorescence spectroscopy of blood plasma and cerebrospinal fluid in diagnosing cerebral gliomas. Key findings included a decreased ratio of tyrosine to tryptophan autofluorescence in the blood plasma of glioma patients compared to a control group with traumatic brain injuries. Furthermore, upon excitation at 320 nm, the plasma fluorescence spectra of Grade IV glioma patients exhibited a long-wavelength shift relative to lower-grade tumors. Additionally, analysis of cerebrospinal fluid fluorescence decay kinetics at 280 nm excitation successfully differentiated glioma patients from the control cohort.

Keywords: autofluorescence spectroscopy, time resolved fluorescence spectroscopy, blood plasma, cerebrospinal fluid.

Received: 27.01.2025
Revised: 05.02.2025
Accepted: 07.04.2025

DOI: 10.61011/OS.2025.05.60794.31-25

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