Proceedings of The XXVIII Annual International Conference "Saratov Fall Meeting 2024", September 23-27, 2024
Biophotonics

Mapping and quantification of microcirculation features during photodynamic therapy

A. V. Guryleva^a, T. G. Grishacheva^b, A. S. Machikhin^a, D. A. Ziganurova^ac, A. S. Belyaeva^a, A. A. Zolotukhina^a, S. G. Chefu^b, P. V. Luzhnov^c, N. N. Petrishchev<sup>b

a</sup> Scientific and Technological Centre of Unique Instrumentation, Russian Academy of Sciences
^b St. Petersburg State Pavlov Medical University
^c Bauman Moscow State Technical University

Abstract: Photodynamic therapy is a promising and minimally invasive treatment option for a wide range of skin diseases. The feasibility of mapping and quantifying blood microcirculation parameters simultaneously in the tumor and surrounding tissues during photodynamic therapy using photoplethysmography was investigated to optimize treatment protocols and assess effectiveness. An experimental setup and digital data processing algorithms were developed and outlined. The mapping of changes in back-scattered light intensity and pulse wave parameters related to tissue blood volume and oxygenation was demonstrated in a model animal. The time dependencies of microcirculation features in healthy and tumor tissues during photoactivation and the subsequent recovery period were presented. The limitations of the proposed approach and strategies to address them are described.

Keywords: biophotonics, noninvasive diagnostics, photodynamic therapy, photoplethysmography, digital image processing.

Received: 01.02.2025
Revised: 10.02.2025
Accepted: 07.04.2025

DOI: 10.61011/OS.2025.05.60792.29-25

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