Proceedings of The XXV Annual International Conference "Saratov Fall Meeting 2021", September 27-October 1, 2021, Saratov, Russia
Biophotonics

Evaluation of the influence of chromium and zinc ions on the collagenolysis process in solutions by the method of dynamic light scattering

A. V. Petrova, A. V. Sidorova, I. A. Sergeeva, G. P. Petrova

Lomonosov Moscow State University

Abstract: Using the method of dynamic light scattering, it was found that the rate of collagen cleavage under the influence of bacterial collagenase decreases by almost 4 times when protein molecules are doped with Cr$^{3+}$ ions, and when the enzyme activator of Zn$^{2+}$ ions is added, it increases by 1.3 times. Based on the experimental data, the reaction rate constants $k_1$ were calculated. It was found that when collagen molecules are tanned with a chromium salt, it becomes more resistant to degradation in solutions containing zinc ions than in solutions with the addition of calcium ions, as we described in earlier works.

Keywords: collagen type 1, bacterial collagenase, chromium chloride, zinc chloride, dynamic light scattering method, translational diffusion coefficient, hydrodynamic radius, reaction rate constant, collagenolysis, clostridium histolyticum.

Received: 20.12.2021
Revised: 10.01.2022
Accepted: 23.03.2022

DOI: 10.21883/OS.2022.06.52624.23-22

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