This article is cited in 1 paper

Conference "Ultrafast Optical Phenomena (Ultra fast Light-2023)" October 2-4, 2023, Lebedev Institute of Physics, Russian Academy of Sciences
Biophotonics

Laser engineering of microbial systems: a new tool for microbiology

N. V. Minaev^a, V. S. Zhigarkov^a, V. S. Cheptsov^ab, V. I. Yusupov<sup>a

a</sup> Institute of Photonic Technologies, Federal Research Centre ‘Crystallography and Photonics’, Russian Academy of Sciences
^b Lomonosov Moscow State University, Faculty of Soil Science

Abstract: One of the new areas of laser bioprinting is laser engineering of microbial systems (LEMS). This technology involves controlled transfer of gel microdroplets containing microorganisms from a donor substrate to acceptor media using a nanosecond laser pulse. During such transfer, living systems are affected by various physical factors: radiation, shock waves, temperature surges. The work carried out a study of the effect on Escherichia coli cells of nanoparticles that are formed during the destruction of a thin gold absorbent coating of the donor plate. It has been shown that the sizes of these nanoparticles, their concentration in the colloid, and the zeta-potential depend significantly on the laser pulse energy. It has been established that Au nanoparticles have a certain effect on the kinetics of microbial growth. A systematization of the main physical factors influencing microorganisms during their laser-induced spatial transfer has been carried out, and the most important scientific results from a practical point of view obtained using promising LEMS technology have been analyzed.

Keywords: laser bioprinting, laser engineering of microbial systems, LEMS, direct laser-induced transfer, microbiology, impact factors.

Received: 11.12.2023
Revised: 09.01.2024
Accepted: 16.01.2024

DOI: 10.61011/OS.2024.01.57557.16-24

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