Communications

Dependence of the solute retention on the column pressure in reversed-phase HPLC

V. I. Deineka^a, A. N. Chulkov^b, I. P. Blinova<sup>a

a</sup> Belgorod State National Research University, Belgorod, Russian Federation
^b Belgorog Branch of FGBU ‘Center for Assessment of Grain Quality’, Belgorod, Russian Federation

Abstract: In the course of reversed-phase HPLC analysis, increase in pressure at the column inlet can affect the retention of sorbates only when they penetrate into the grafted phase. Due to conformational changes in the alkyl groups of the loose grafted layer, the solvation of sorbates by these alkyl groups would increase, leading to a growth of the retention factors. In the case of the surface sorption of solutes, the effect is not observed.

Keywords: reversed-phase HPLC, retention, anthocyanins, pressure dependence, mechanism of retention, ODS stationary phase structure.

Language: English

DOI: 10.1016/j.mencom.2023.04.044

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