R. Kh. Dzhenloda, D. G. Petrov, V. M. Shkinev, B. Ya. Spivakov, “DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection”, Mendeleev Commun., 2017, Volume 27, Issue 3,Pages <nobr>302

This article is cited in 5 papers

Communications

DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection

R. Kh. Dzhenloda^a, D. G. Petrov^b, V. M. Shkinev^a, B. Ya. Spivakov^a

^a V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
^b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russian Federation

Abstract: A new method is proposed for dynamic DNA extraction from environmental samples (soil, water) on suspension columns containing silica-coated magnetic sorbents (Fe₃O₄@SiO₂) under a combined influence of ultrasound and magnetic fields. The recovered DNA was subsequently determined by real-time polymerase chain reaction on an ANK-32 device. It has been shown for the first time that the suspension columns enable the preconcentration of DNA (taking Mycobacterium tuberculosis as an example) in a dynamic mode from large volumes of water and soil extracts (100 ml and more) to be performed.

Language: English

DOI: 10.1016/j.mencom.2017.05.029