This article is cited in 11 papers

Communications

Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC

R. R. Alieva, E. G. Zavyalova, V. N. Tashlitsky, A. M. Kopylov

Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russian Federation

Abstract: A quantitative technique for estimation of the oligomeric state of G-quadruplexes has been developed for the first time. The simplest 15-mer G-quadruplex antithrombin aptamer HD1 and its analogues, namely RA36 as a covalent dimer of HD1 as well as GL2–HD1 as a non-covalent dimer with G-quadruplex lock, were used as model objects. HD1 and RA36 have been shown to be single-stranded G-quadruplexes, whereas GL2–HD1 forms non-covalent dimers (GL2–HD1)₂ in a concentration-dependent manner, affording ∼50% of the dimers at 1mmoldm⁻³ concentration.

Language: English

DOI: 10.1016/j.mencom.2019.07.023

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