Application of optical methods in standardization of collagen-containing hydrogen for 3D bioprinting of supporting and connective tissues

Elena V. Timchenko^a, Nikolai A. Rjabov^b, Oleg O. Frolov^a, Pavel E. Timchenko^a, Larisa T. Volova^b, Sergej S. Ivanov<sup>a

a</sup> Institute of Informatics and Cybernetics, Samara University, Samara, Russian Federation
^b Samara State Medical University, Samara, Russian Federation

Abstract: The paper presents the results of the application of optical methods in the standardization of collagen-containing allogeneic hydrogel produced at the Biotech Research Institute (Samara, Russia) from bioimplants of the Lioplast® trademark in comparison with the hydrogels available on the market from Rokit and Cellink companies. The Raman spectroscopy method was used as the main research method. An additional research method was the method of IR Fourier spectroscopy.As a result of the conducted research using optical methods, it was found that the collagen structure is completely preserved in the composition of the allogeneic hydrogel produced at the Biotech Research Institute (Samara, Russia). Hydrogels of imported Rokit and Cellink companies also have a similar spectral composition.The obtained results can be further used as a rapid assessment and standardization of collagen-containing hydrogel with the addition of various components for personalized 3D bioprinting of human supporting and connective tissues.As part of the current import substitution task, the developed collagen-containing allogeneic hydrogel may in the future represent a competitive analogue to foreign commercial products — hydrogels in bioprinting.

Keywords: Raman spectroscopy, infrared Fourier spectroscopy, collagen-containing hydrogel, supporting and connective tissues, 3D bioprinting.

UDC: 543.424

Received: 18.10.2024
Received in revised form: 23.11.2024
Accepted: 10.02.2025

Language: English