This article is cited in 2 papers

ATOMS, SPECTRA, RADIATIONS

Localized donor-acceptor pairs of fluorophores: Determination of the energy transfer rate by nonlinear fluorimetry

E. A. Shirshin, A. A. Banishev, V. V. Fadeev

M. V. Lomonosov Moscow State University, Faculty of Physics

Abstract: A laser fluorescence method has been developed for determining the energy transfer rate. It is based on the model of collective states of localized donor-acceptor pairs of fluorophores proposed in our previous work and involves nonlinear fluorimetry (other photophysical parameters of the donor and acceptor are simultaneously determined). The proposed approach has been tested on mRFP1 fluorescent (red) protein.

PACS: 33.15.Hp, 42.62.Fi

Received: 06.04.2009

 English version: Journal of Experimental and Theoretical Physics Letters, 2009, 89:10, 475–478

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